POC Kenya 2012 Hands-on Demos

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POC Kenya Diagnostic Demonstrations

General Info...

Marc Dusseiller - DIY Microscopy

POC Kenya diy microscope.JPG

Abstract

This project deals with simple ways to build microscopes out of cheap and easily accessible devices/parts. A small hack to the optics of a standard webcam allows to create video data, with a magnification of around 100 to 400x (depending on the model and the setup) at a working distance of a few mm. The highest magnifications can be achieved by inverting (putting upside down) the lens. Addition of good lighting by the use of leds allows to create images using a bright-field method (shine through the stuff) or dark field method (shine from the side and look at the reflections and scattering). To achieve a suitable and stable setup a mechanical stage can be built from plywood, plastic or cardboard.

more info on Marc Dusseiller - DIY Microscopy

Links

Hackteria | Open Source Biological Art


John Example - PaperSmaper

Microfluidics.jpg

Abstract

more info on John Example - PaperSmaper

Links

Joseph Ndung'u - LAMP

Microfluidics.jpg

Other Instructions

PDMS preparation

Microfluidics.jpg

we developed a number of prototypes by casting of a transparent silicone rubber. Polydimethylsiloxan PDMS, which can be ordered from dow corning, product name is sylgard 184.

Crayon PADs:

Microfluidics.jpg

This is an alternative to wax printing that is crude but fast--good for prototyping.

1) Draw the desired lanes or spots on absorbent paper using an ordinary wax crayon. The type of paper is not critical; you can use Whatman #1 filter paper for spot tests, or a fast chromatography paper for lane tests. Or you can use a paper towel. In most cases, it works best to put the lanes on both front and back sides of the paper.

2) Bake in an oven at 100C for about 3 min. You can test to see if the wax has soaked through the paper by adding a drop of food coloring next to a line and seeing if any bleeds through.

--Marya Lieberman


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